PathHunter® CXCR2 (IL8RB) Activated GPCR Internalization U2OS Cell Line

The PathHunter® CXCR2 (IL8RB) Activated GPCR Internalization U2OS Cell Line measures CXCR2 (IL8RB) (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0934C3 Size: 2 vials

Inquire

Biological Information
Background Information:	The PathHunter® CXCR2 (IL8RB) Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CXCL8/IL-8) based internalization of CXCR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR2 (IL8RB). Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR2 (IL8RB)-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Chemokine Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_001557
Target Name:	CXCR2 (IL8RB)
Target Aliases:	Chemokine (C-X-C motif) receptor 2, CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB, WHIMS2
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®