PathHunter® CXCR2 (IL8RB) Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® CXCR2 (IL8RB) Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CXCL8/IL-8) based internalization of CXCR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR2 (IL8RB). Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR2 (IL8RB)-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR2 (IL-8RB)

Protein Aliases:

CXCR-2|CDw128b|GRO/MGSA receptor|High affinity interleukin-8 receptor B|IL-8R B|IL-8 receptor type 2|CD antigen CD182

Accession Number:

NM_001557

UniProt Number:

P25025

Gene Name:

CXCR2

Gene ID:

3579

Gene Aliases:

CMKAR2|CD182

Target Species:

Human

Cell Background:

U2OS