PathHunter® AVPR1A Total GPCR Internalization U2OS Cell Line

The PathHunter® AVPR1A Total GPCR Internalization U2OS Cell Line measures AVPR1A (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0751C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® AVPR1A Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. vasopressin) based internalization of AVPR1A via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged AVPR1A and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the AVPR1A receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Vasopressin & Oxytocin Receptors
Sub Family:	Class A
Coupling:	Gq
Accession Number:	NM_000706.3
Target Name:	AVPR1A
Target Aliases:	arginine vasopressin receptor 1A, V1aR, AVPR1, AVPR V1a
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®