PathHunter® Human AVPR1A Stable Cell Line Total GPCR Internalization Assay (U2OS)

Background Information:

The PathHunter Human AVPR1A Stable Cell Line Total GPCR Internalization Assay (U2OS) utilizes the AVPR1A Total GPCR Internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., vasopressin)- based internalization of AVPR1A via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged AVPR1A and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, the resulting internalization of the AVPR1A receptor to the early endosome leads to production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

V1A

Protein Aliases:

AVPR V1a|Antidiuretic hormone receptor 1a|Vascular/hepatic-type arginine vasopressin receptor

Accession Number:

NM_000706.3

UniProt Number:

P37288

Gene Name:

AVPR1A

Gene ID:

552

Gene Aliases:

AVPR1

Target Species:

Human

Cell Background:

U2OS