S1P3 (EDG3) Gq Stable Cell Line Calcium Signaling Assay (CHO-K1)

Background Information:

The S1P3 (EDG3) Gq Stable Cell Line Calcium Signaling Assay (CHO-K1) utilizes the EDG3 Gq cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that target GPCRs. This cell line enables the assessment of ligand (e.g., S1P) based activation of EDG3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDG3, a naturally Gq coupled GPCR. Upon activation of EDG3, the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Coupling:

Gq

Protein Name:

S1P3

Protein Aliases:

EDG3|Endothelial differentiation G-protein coupled receptor 3|Lysophospholipid receptor B3|Sphingosine 1-phosphate receptor Edg-3|S1P receptor Edg-3

Accession Number:

NM_005226

UniProt Number:

Q99500

Gene Name:

S1PR3

Gene ID:

1903

Gene Aliases:

EDG-3

Target Species:

Human

Cell Background:

CHO-K1