Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. lymphotactin) based activation of XCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress XCR1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of XCR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

XCR1 (GPR5)

Protein Aliases:

Lymphotactin receptor|XC chemokine receptor 1

Accession Number:

NM_005283.

UniProt Number:

P46094

Gene Name:

XCR1

Gene ID:

2829

Target Species:

Human

Cell Background:

Chem-4