Ready-to-Assay GPR68/OGR1 Proton-Sensing Receptor Frozen Cells

Background Information:

The Ready-to-Assay GPR68/OGR1 Proton-Sensing Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses pH based activation of GPR68 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GPR68 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GPR68 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

GPR68

Accession Number:

NM_003485

UniProt Number:

Q15743

Gene Name:

GPR68

Gene ID:

8111

Gene Aliases:

OGR1

Target Species:

Human

Cell Background:

Chem-1