Ready-to-Assay VPAC2 Receptor Frozen Cells

Background Information:

The Ready-to-Assay VPAC2 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress VIPR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of VIPR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Protein Name:

VIP2 (VPAC2)

Protein Aliases:

VIP and PACAP receptor 2

Accession Number:

NM_003382

UniProt Number:

P41587

Gene Name:

VIPR2

Gene ID:

7434

Gene Aliases:

VPAC2|VPAC2R

Target Species:

Human

Cell Background:

Chem-1