Ready-to-Assay S1P3 Lysophospholipid Receptor Frozen Cells

Background Information:

The Ready-to-Assay S1P3 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress S1PR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Protein Name:

S1P3

Protein Aliases:

EDG3|Endothelial differentiation G-protein coupled receptor 3|Lysophospholipid receptor B3|Sphingosine 1-phosphate receptor Edg-3|S1P receptor Edg-3

Accession Number:

NM_005226

UniProt Number:

Q99500

Gene Name:

S1PR3

Gene ID:

1903

Gene Aliases:

EDG-3

Target Species:

Human

Cell Background:

Chem-1