Ready-to-Assay GIP Glucagon Receptor Frozen Cells

Background Information:

The Ready-to-Assay GIP Glucagon Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GIPR alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GIPR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GIP

Protein Aliases:

Glucose-dependent insulinotropic polypeptide receptor

Accession Number:

NM_000164

UniProt Number:

P48546

Gene Name:

GIPR

Gene ID:

2696

Target Species:

Human

Cell Background:

Chem-9