Ready-to-Assay CCR2B Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCR2B Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. eotaxin) based activation of CCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CCR2

Protein Aliases:

CC-CKR-2|CCR-2|CCR2|Monocyte chemoattractant protein 1 receptor|MCP-1-R|CD antigen CD192

Accession Number:

NM_001123396

UniProt Number:

P41597

Gene Name:

CCR2

Gene ID:

729230

Gene Aliases:

CC-CKR-2|CKR2|MCP-1-R|CD192|FLJ78302

Target Species:

Human

Cell Background:

Chem-1