Ready-to-Assay CaS Calcium Sensor Receptor Frozen Cells

Background Information:

The Ready-to-Assay CaS Calcium Sensor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. cannabinoids, CP 55,940) based activation of CASR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CASR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CASR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Calcium-Sensing

Sub Family:

Class C

Protein Name:

CaS

Protein Aliases:

severe neonatal hyperparathyroidism

Accession Number:

NM_000388

UniProt Number:

P41180

Gene Name:

CASR

Gene ID:

846

Gene Aliases:

FHH|NSHPT|GPRC2A

Target Species:

Human

Cell Background:

Chem-1