PathHunter® Human PR beta Stable Cell Line Nuclear Translocation Assay (U2OS)

Background Information:

The PathHunter Human PR beta Stable Cell Line Nuclear Translocation Assay (U2OS) utilizes the PRβ Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates affecting NHR activation and nuclear translocation. This cell line enables assessment of ligand (e.g. testosterone, norgestrel) based NHR activation via detection of resulting PRβ nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged PRβ NHR and nuclear localized EA. Upon activation of PRβ, induced nuclear translocation leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

NHR

Family:

3-Ketosteroid

Sub Family:

Steroid

Protein Name:

PR

Protein Aliases:

Nuclear receptor subfamily 3 group C member 3

Accession Number:

NM_000926

UniProt Number:

P06401

Gene Name:

PGR

Gene ID:

5241

Gene Aliases:

PR|NR3C3

Target Species:

Human

Cell Background:

U2OS