Biological Information
Background Information:
The PathHunter® U2OS INSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated INSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
RTK
Sub Family:
Protein Tyrosine
Protein Name:
IR
Protein Aliases:
CD antigen CD220 [Cleaved into: Insulin receptor subunit alpha; Insulin receptor subunit beta]
Accession Number:
NM_000208
UniProt Number:
P06213
Gene Name:
INSR
Gene ID:
3643
Gene Aliases:
CD220
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
PathHunter®