PathHunter® SREBP1c Nuclear Translocation Cell Line (U2OS)

Background Information:

PathHunter® Nuclear Translocation cell lines are engineered to co-express two proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA), which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-express an untagged secondary protein involved in the pathway of interest.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

SREBF1

Protein Aliases:

Sterol regulatory element-binding protein 1

Accession Number:

NP_001308025.1

UniProt Number:

P36956

Gene Name:

SREBF1

Gene ID:

6720

Gene Aliases:

SREBP1|bHLHd1|SREBP-1c|SREBP1a

Target Species:

Human

Cell Background:

U2OS