Background

Vascular Endothelial Growth Factor (VEGF)-A is a key protein that regulates angiogenesis and vascular permeability. It is elevated in diseases like diabetic retinopathy, macular edema, and glaucoma. Ranibizumab is a 48 kD humanized monoclonal antibody fragment (Fab) derived from bevacizumab that binds VEGF-A, blocking its interaction with VEGF receptors on endothelial cells. This prevents VEGFR-2 activation and downstream signaling, inhibiting endothelial cell proliferation, reducing vascular leakage, and blocking the formation of abnormal blood vessels in the eye.

PathHunter Ranibizumab Bioassay Kits model this molecular MOA in a simple, homogenous format, suitable for implementation in characterization, comparability studies, potency testing in QC lot release, stability program, and detection of neutralizing antibodies.

Product Highlights
  • Cell-based functional assay for anti-VEGF drugs, Ranibizumab or Biosimilar based on their mechanism of action
  • Simple, homogenous protocol with results in less than 24 hours
  • Significantly better assay performance compared to difficult HUVEC assay
  • Suitable for rank ordering, comparability studies, potency measurement, stability studies and neutralizing antibody detection
  • Applicable across various stages from characterization and QC lot-release

Assay Principle

The PathHunter Ranibizumab bioassay utilizes EFC-based platform and is designed to detect VEGF-A-induced functional homodimerization of the kinase insert domain (KDR), also known as the VEGFR2 receptor. Activation of the KDR receptor through VEGF-A leads to receptor dimerization, which is an essential event in the receptor's signaling cascade. A. The PathHunter KDR/KDR bioassay cells have been engineered to co-express KDR fused to PK, and another KDR construct fused to EA. B. Upon activation by VEGF-A, the VEGFR2 receptors naturally dimerize forcing the two fragments to complement and create an active β-gal enzyme. Active β-gal hydrolyzes its substrate and produces a chemiluminescent signal, indicating receptor activation C. Anti-VEGF antibodies like ranibizumab, inhibit VEGF-A’s ability to activate the receptors resulting in a dose-dependent reduction of the chemiluminescent signal.

Ranibizumab Bioassay Kit - Assay Principle