PathHunter® Mouse RHO(P23H) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Mouse RHO(P23H) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS) utilizes the U2OS mouse RHO(P23H) ENDO-EA Pharmacotrafficking cell line. This stable engineered cell line is designed for use in studying pharmacochaperones targeting mouse RHO. This cell line enables assessment of chaperone-based stabilization of mouse RHO(P23H) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant mouse RHO(P23H), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant mRHO, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Opsin

Sub Family:

Orphan and other 7TM

Coupling:

Gt

Protein Name:

OPSD

Accession Number:

NM_145383.1

UniProt Number:

P15409

Gene Name:

RHO

Gene ID:

212541

Target Species:

Mouse

Cell Background:

U2OS