PathHunter® Mouse MCHR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Mouse MCHR1 β-Arrestin Cell-Based Assay Kit (CHO-K1) utilizes the Mouse MCHR1 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., MCH) based activation of mouse MCHR1 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged mouse MCHR1 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Melanin-Concentrating Hormone

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

MCH1

Protein Aliases:

MCH-R1|MCHR-1|G-protein coupled receptor 24|MCH-1R|MCH1R|MCHR|SLC-1|Somatostatin receptor-like protein

Accession Number:

NM_145132

UniProt Number:

Q8JZL2

Gene Name:

MCHR1

Gene ID:

207911

Gene Aliases:

SLC1|MCH1R

Target Species:

Mouse

Cell Background:

CHO-K1