PathHunter® Mouse MC4R β-Arrestin Stable Cell Pool Assay (CHO-K1)

Background Information:

The PathHunter Mouse MC4R β-Arrestin Stable Cell Pool Assay (CHO-K1) utilizes the Mouse MC4R β-Arrestin stable cell pool. This stable cell pool is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. The included cells enable the assessment of ligand (e.g., α-MSH) based activation of mouse MC4R GPCR activity via detection of β-arrestin recruitment. This cell pool employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged mouse MC4R target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell pool (supplied as two vials of cryopreserved cells); cell pool specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell pool, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell pools in accordance with the cell pool license agreed to upon purchase of this cell pool assay kit.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Protein Name:

MC4

Accession Number:

NM_016977.1

UniProt Number:

P56450

Gene Name:

Mc4r

Gene ID:

17202

Target Species:

Mouse

Cell Background:

CHO-K1