PathHunter® Human TrkA-P75 Stable Cell Line Functional Kinase Assay (U2OS)

Background Information:

The PathHunter Human TrkA-P75 Stable Cell Line Functional Kinase Assay (U2OS) utilizes the U2OS TrkA-P75 Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation by detecting the recruitment of SH2 domain proteins to activated human TrkA-p75 dimers. The included cell line overexpresses PK-tagged TrkA, untagged P75, and an EA-tagged SH2 domain protein. Upon ligand (e.g. βNGF)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKA

Protein Aliases:

TRK1-transforming tyrosine kinase protein|Tropomyosin-related kinase A|Tyrosine kinase receptor|Tyrosine kinase receptor A|Trk-A|gp140trk|p140-TrkA|high affinity nerve growth factor receptor

Accession Number:

NM_002529

UniProt Number:

P04629

Gene Name:

NTRK1

Gene ID:

4914

Gene Aliases:

TRK|TRKA|MTC

Target Species:

Human

Cell Background:

U2OS