PathHunter® Human TORC3 (CRTC3) Nuclear Translocation Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human TORC3 (CRTC3) Nuclear Translocation Cell-Based Assay Kit (U2OS) utilizes the U2OS TORC3 Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates affecting the CRTC/CREB pathway. This cell line enables assessment of ligand (e.g. forskolin) based pathway activation via detection of resulting TORC3 (CRTC3) nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged TORC3 (CRTC3) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC3 leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

CRTC3

Accession Number:

NM_022769.4

UniProt Number:

Q6UUV7

Gene Name:

CRTC3

Gene ID:

64784

Gene Aliases:

FLJ21868

Target Species:

Human

Cell Background:

U2OS