PathHunter® Human RAR alpha Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human RAR alpha Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1) utilizes the RARα Protein Interaction cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. retinoic acid) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged RARα NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of RARα, induced interaction with SRCP leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

NHR

Family:

Retinoic Acid

Sub Family:

Non-Steroid

Protein Name:

RARalpha

Protein Aliases:

Nuclear receptor subfamily 1 group B member 1

Accession Number:

NM_000964

UniProt Number:

P10276

Gene Name:

RARA

Gene ID:

5914

Gene Aliases:

RAR|NR1B1

Target Species:

Human

Cell Background:

CHO-K1