PathHunter® Human IL-10 (IL10RA/IL10RB) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-10 (IL10RA/IL10RB) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL10RA/IL10RB Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-10)-induced dimerization of IL10RA/IL10RB pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL10RA fused to PK and IL10RB fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This Kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Cytokine Receptor

Family:

IL-10 Receptor

Protein Name:

IL10RA

Protein Aliases:

IL-10R subunit alpha|IL-10RA|CDw210a|Interleukin-10 receptor subunit 1|IL-10R subunit 1|IL-10R1|CD antigen CD210

Accession Number:

NM_001558.3;

UniProt Number:

Q13651

Gene Name:

IL10RA

Gene ID:

3587

Gene Aliases:

IL10R

Target Species:

Human

Cell Background:

U2OS