PathHunter® Human EphB4 Functional Kinase Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human EphB4 Functional Kinase Cell-Based Assay Kit (U2OS) utilizes the U2OS EphB4 Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of EphB4 activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human EphB4 and an EA-tagged SH2 domain protein. Upon ligand (e.g. ephrin B2)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphB4

Protein Aliases:

Hepatoma transmembrane kinase|Tyrosine-protein kinase TYRO11

Accession Number:

NM_004444

UniProt Number:

P54760

Gene Name:

EPHB4

Gene ID:

2050

Gene Aliases:

Tyro11

Target Species:

Human

Cell Background:

U2OS