PathHunter® Human AR Stable Cell Line Nuclear Translocation Assay (HEK 293)

Background Information:

The PathHunter Human AR Stable Cell Line Nuclear Translocation Assay (HEK 293) utilizes the AR Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates affecting NHR activation and nuclear translocation. This cell line enables assessment of ligand (e.g. testosterone, fluorotestosterone) based NHR activation via detection of resulting AR nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged AR NHR and nuclear localized EA. Upon activation of AR, induced nuclear translocation leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

NHR

Family:

3-Ketosteroid

Sub Family:

Steroid

Protein Name:

AR

Protein Aliases:

testicular feminization|Kennedy disease

Accession Number:

NM_000044

UniProt Number:

P10275

Gene Name:

AR

Gene ID:

367

Gene Aliases:

AIS|NR3C4|SMAX1|HUMARA

Target Species:

Human

Cell Background:

HEK293