PathHunter® F2RL3 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® F2RL3 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. serine proteases, protease-activated receptor 4) based internalization of F2RL3 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged F2RL3. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting F2RL3-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Proteinase-Activated

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

PAR4

Protein Aliases:

Coagulation factor II receptor-like 3|Thrombin receptor-like 3

Accession Number:

AY431102

UniProt Number:

Q96RI0

Gene Name:

F2RL3

Gene ID:

9002

Gene Aliases:

PAR4

Target Species:

Human

Cell Background:

U2OS