PathHunter® eXpress SREBP2 Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress SREBP2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the cholesterol biosynthesis pathway. This kit assesses ligand (e.g. 5'-UMP) based pathway activation via detection of resulting SREBP2 nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged SREBP2 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of SREBP2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

SREBF2

Protein Aliases:

Class D basic helix-loop-helix protein 2|bHLHd2|Sterol regulatory element-binding transcription factor 2 [Cleaved into: Processed sterol regulatory element-binding protein 2]

Accession Number:

NM_004599.2

UniProt Number:

Q12772

Gene Name:

SREBF2

Gene ID:

6721

Gene Aliases:

BHLHD2|SREBP2

Target Species:

Human

Cell Background:

U2OS