PathHunter® eXpress PTGIR Total GPCR Internalization Assay

Background Information:

The PathHunter® PTGIR Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. prostaglandin I2) based internalization of PTGIR via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gs

Protein Name:

IP

Protein Aliases:

PGI receptor|PGI2 receptor|Prostanoid IP receptor

Accession Number:

AY242134

UniProt Number:

P43119

Gene Name:

PTGIR

Gene ID:

5739

Gene Aliases:

IP|PRIPR

Target Species:

Human

Cell Background:

U2OS