PathHunter® Epoetin Alfa Bioassay Kit

Background Information:

The PathHunter® Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoietin or Epoetin Alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The included cells overexpress EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. This assay has been optimized and qualified with Aranesp® (not supplied in the bioassay kit). Aranesp® is a registered trademark of Amgen Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

Cytokine Receptor

Family:

Prolactin Receptor

Protein Name:

EPOR

Accession Number:

NM_000121.3

UniProt Number:

P19235

Gene Name:

EPOR

Gene ID:

2057

Target Species:

Human

Cell Background:

U2OS