Biological Information

Background Information:

The PathHunter® mHCRTR1 Cell Pool is a stable, engineered cell pool for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. orexin A) based activation of mHCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell pool overexpresses ProLink-tagged mHCRTR1 and EA-tagged β-Arrestin-2. Activation of mHCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable cell pool is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Protein Name:

OX1

Protein Aliases:

Orexin receptor 1

Accession Number:

NM_198959

UniProt Number:

P58307

Gene Name:

Hcrtr1

Gene ID:

230777

Gene Aliases:

OX1R|OXR1|ORXR1

Target Species:

Mouse

Cell Background:

CHO-K1

Usage

Product Type:

Cell Pool

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®