Biological Information

Background Information:

The PathHunter® mCNR1 (CB1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CP 55,940) based activation of mCNR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mCNR1 and EA-tagged β-Arrestin 2. Activation of mCNR1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein.

Target Class:

GPCR

Family:

Cannabinoid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CB1

Protein Aliases:

CB1|CANN6

Accession Number:

NM_007726

UniProt Number:

P47746

Gene Name:

CNR1

Gene ID:

12801

Gene Aliases:

CB1K5|CB-R|CB1|CANN6|CB1A

Target Species:

Mouse

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

CNS/Addiction/Pain

Additional Information

Brand:

PathHunter®