PathHunter® ADCYAP1R1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® ADCYAP1R1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. PACAP) based internalization of ADCYAP1R1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Coupling:

Gq & Gs

Protein Name:

PAC1 (PACAP)

Protein Aliases:

PACAP-R-1|PACAP-R1|PACAP receptor 1

Accession Number:

AY366498; NM_001118

UniProt Number:

P41586

Gene Name:

ADCYAP1R1

Gene ID:

117

Gene Aliases:

PAC1|PACAPR|PAC1R

Target Species:

Human

Cell Background:

U2OS