InCELL Hunter™ Human PIKFYVE Stable Cell Line Target Engagement Assay (HEK 293)

Background Information:

The InCELL Hunter Human PIKFYVE Stable Cell Line Target Engagement Assay (HEK 293) utilizes the PIKFYVE Target Engagement cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PIKFYVE via detection of PIKFYVE levels. This product uses EFC technology. The cell line included overexpresses PIKFYVE's kinase domain fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded, and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

1-PtdIns 5-Kinase

Sub Family:

Lipid

Protein Name:

PIKFYVE

Protein Aliases:

FYVE finger-containing phosphoinositide kinase|PIKfyve|Phosphatidylinositol 3-phosphate 5-kinase type III|PIPkin-III|Type III PIP kinase

Accession Number:

NM_015040.3

UniProt Number:

Q9Y2I7

Gene Name:

PIKFYVE

Gene ID:

200576

Gene Aliases:

KIAA0981|PIP5K3

Target Species:

Human

Cell Background:

HEK293