Biological Information

Background Information:

The ChemiSCREEN™ OX1 Orexin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. orexins) based activation of HCRTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCRTR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HCRTR1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Protein Name:

OX1

Protein Aliases:

orexin receptor 1

Accession Number:

NM_001525

UniProt Number:

O43613

Gene Name:

HCRTR1

Gene ID:

3061

Gene Aliases:

OX1R

Target Species:

Human

Cell Background:

Chem-1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Fluorescence

Additional Information

Brand:

ChemiSCREEN™