ChemiSCREEN™ OX1 Orexin Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ OX1 Orexin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. orexins) based activation of HCRTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCRTR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HCRTR1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Protein Name:

OX1

Protein Aliases:

orexin receptor 1

Accession Number:

NM_001525

UniProt Number:

O43613

Gene Name:

HCRTR1

Gene ID:

3061

Gene Aliases:

OX1R

Target Species:

Human

Cell Background:

Chem-1