Biological Information
Background Information:
The ChemiBRITE GLP-1 Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GLP-1, exenatide) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Glucagon
Sub Family:
Class B
Protein Name:
GLP-1
Protein Aliases:
GLP-1-R|GLP-1R
Accession Number:
NM_002062
UniProt Number:
P43220
Gene Name:
GLP1R
Gene ID:
2740
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
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Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
ChemiBRITE