ChemiBRITE GLP-1 Receptor Stable Cell Line

Background Information:

The ChemiBRITE GLP-1 Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GLP-1, exenatide) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

HEK293