cAMP Hunter™ Human RXFP3 Gi Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Human RXFP3 Gi Cell-Based Assay Kit (CHO-K1) utilizes the Human RXFP3 cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of decreasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type RXFP3, a naturally Gi/Go coupled GPCR. Upon activation of RXFP3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Relaxin Family Peptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

RXFP3

Protein Aliases:

G protein-coupled receptor SALPR|G-protein coupled receptor GPCR135|Relaxin family peptide receptor 3|Somatostatin- and angiotensin-like peptide receptor

Accession Number:

NM_016568.2

UniProt Number:

Q9NSD7

Gene Name:

RXFP3

Gene ID:

51289

Gene Aliases:

RLN3R1|SALPR

Target Species:

Human

Cell Background:

CHO-K1