cAMP Hunter™ eXpress GPR109B CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR109B, a naturally Gi/Go coupled GPCR. Upon activation of GPR109B, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

GPR109B

Protein Aliases:

G-protein coupled receptor HM74|G-protein coupled receptor HM74B|Niacin receptor 2|Nicotinic acid receptor 2|HCA3

Accession Number:

NM_006018

UniProt Number:

P49019

Gene Name:

HCAR3

Gene ID:

8843

Gene Aliases:

GPR109B|HCA3|HM74B|NIACR2

Target Species:

Human

Cell Background:

CHO-K1