Ready-to-Assay MCH1 Melanin-Concentrating Hormone Receptor Frozen Cells

Background Information:

The Ready-to-Assay MCH1 Melanin-Concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MCHR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Melanin-Concentrating Hormone

Sub Family:

Class A

Protein Name:

MCH1

Protein Aliases:

MCH-R1|MCHR-1|G-protein coupled receptor 24|MCH-1R|MCH1R|MCHR|SLC-1|Somatostatin receptor-like protein

Accession Number:

AY562945

UniProt Number:

Q99705

Gene Name:

MCHR1

Gene ID:

2847

Gene Aliases:

SLC1|MCH1R

Target Species:

Human

Cell Background:

Chem-1