Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells

Background Information:

The Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MC5R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Protein Name:

MC5

Protein Aliases:

MC-2

Accession Number:

NM_005913

UniProt Number:

P33032

Gene Name:

MC5R

Gene ID:

4161

Target Species:

Human

Cell Background:

Chem-10