Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP3

Protein Aliases:

PGE2 receptor EP3 subtype|PGE2-R|Prostanoid EP3 receptor

Accession Number:

NM_198716

UniProt Number:

P43115

Gene Name:

PTGER3

Gene ID:

5733

Gene Aliases:

EP3

Target Species:

Human

Cell Background:

Chem-1