Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL27) based activation of CCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CCR1

Protein Aliases:

CC-CKR-1|CCR-1|CCR1|HM145|LD78 receptor|Macrophage inflammatory protein 1-alpha receptor|MIP-1alpha-R|RANTES-R|CD antigen CD191

Accession Number:

L09230

UniProt Number:

P32246

Gene Name:

CCR1

Gene ID:

1230

Gene Aliases:

CKR-1|MIP1aR|CD191

Target Species:

Human

Cell Background:

Chem-1