Ready-to-Assay CB2 Cannabinoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay CB2 Cannabinoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. cholecystokinin) based activation of CNR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CNR2 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CNR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Cannabinoid

Sub Family:

Class A

Protein Name:

CB2

Protein Aliases:

CB2|hCB2|CX5

Accession Number:

X74328

UniProt Number:

P34972

Gene Name:

CNR2

Gene ID:

1269

Gene Aliases:

CB2

Target Species:

Human

Cell Background:

Chem-4