PathHunter® eXpress OPRD1 Activated GPCR Internalization Assay

Background Information:

The PathHunter® OPRD1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. DADLE/DPDPE) based internalization of OPRD1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged OPRD1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting OPRD1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

delta (DOP)

Protein Aliases:

DOR-1

Accession Number:

NM_000911

UniProt Number:

P41143

Gene Name:

OPRD1

Gene ID:

4985

Gene Aliases:

OPRD

Target Species:

Human

Cell Background:

U2OS