Knowledge-Based Videos

Enhancing Your Assay Familiarity for Drug Discovery & Development

Knowledge-based, on-demand videos are short video recordings for drug discovery and development scientists covering a variety of topics about Eurofins DiscoverX products, technologies, and capabilities to help you accelerate your drug discovery and development programs with confidence.

Immuno-Oncology

► Novel Cell-Based Assays to Enable Immunotherapy Drug Development for Checkpoint Receptors

Learn about PathHunter® checkpoint receptor assays (cell lines and bioassays) for innate and adaptive immunity.

Assays discussed include functional cell-based assays for immunoglobulin and TNFR superfamilies like CD28, ICOS, PD1, CD47, SIRPα, and OX40 and assays for testing agonist antibodies (clustering and binding assays for bi-specifics). 28 min.

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► A Novel MOA-reflective Bioassay for Quantifying Potency of Therapeutics Targeting the SIRPα|CD47 Signaling Axis

Learn about the first commercially-available SIRPα/CD47 cell-based assay -- PathHunter SIRPα Signaling Assay.

Find out more about the generation of an engineered cell system that measures activation and inhibition of the CD47 / SIRPα signaling axis without using macrophages. 20 min.

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► KILR Platform for Cytotoxicity Applications

Learn how KILR® cytotoxicity assays provide a simple, non-radioactive and dye free method to specifically measure target cell death in a co-culture with effector cells.

Assays for cancer immunotherapy drugs need to detect target cancer cell death when co-cultured with immune effector cells. Radioactive or leaky dyes make it difficult to specifically detect target cell death in a co-culture with immune effector cells. 25 min.

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► Single Donor-Derived KILR CD16 Effector Cells for ADCC and T Cell Redirection Applications

Learn how KILR® CD16 effector cells ensure assay reproducibility with higher signal to background ratios, without worrying about donor variability.

PBMC effector cells have inherent donor variability, reducing their consistency and reproducibility in lot release assays. Antibody-Dependent Cell Cytotoxicity (ADCC) assays that measure cell death often have low signal to background ratios and suffer from this donor variability of the PBMCs. 28 min.

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Bioassays

► Cell Banking for Bioassays

Learn how to confidently de-risk your biologics’ lot release strategy by establishing a dedicated analytical cell banks for your bioassay.

Find out more about Eurofins DiscoverX’s critical reagent strategy that employs a 2-tiered cell bank approach designed to ensure a multi-decade supply of high quality bioassay production lots for biopharmaceutical companies. 21 min.

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Small Molecules

► Detection of Protein Turnover Induced by Targeted Degraders

Learn how to easily detect target protein turnover induced by targeted degrader molecules, such as PROTACs (trademark of Arvinas).

Discover how the cell-based, SPRINTer™ protein turnover biosensor assays can be used to rapidly screen small molecule therapeutics and quantify changes in endogenous protein levels in disease relevant cell models. 20 min.

View Related Products discoverx.com/turnover and Poster Recording ~5 min.

► InCELL Compound-Target Engagement Assays for Drug Discovery

Learn how InCELL cell-based binding assays can be used to detect compound cell entry and engagement to specific targets including enzymes, PROTACs, and epigenetic, plasma membrane, and ER proteins.

These universal assays are designed for screening inhibitors, validating hits identified in biochemical assays, measuring cellular EC50 values, ranking compounds, and more – all in the native cellular environment. 27 min.

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Target Focused

► Accelerate Your COVID-19 Drug Discovery with Qualified Cell-Based Assays for Proinflammatory Cytokines

Learn how functional cell-based assays support COVID-19 drug discovery programs, particularly in managing proinflammatory cytokines associated with high mortality rate in COVID-19 patients.

Listen to several case studies for our target-specific, MOA-reflective cell based assays for small molecules and biologic therapeutics to accelerate your drug development through QC Lot Release programs for COVID-19 research. 24 min.

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► Reporter Assays for Therapeutics Targeting Signaling Pathways

Learn how PathHunter® signaling reporter assays can be used to evaluate the cellular impact of therapeutics targeting a variety of proteins in NFkB, NFAT, and STAT3 signaling pathways.

Utilize these easy-to-use cell-based, reporter gene assays to understanding the MOAs of therapeutics targeting TNFα, PD-1, IL-6, CD4, and RANK receptors. Assays discussed include a comparison of inhibitory checkpoint receptor PD-1 functional data from 2 different cell-based assay types – distal reporter based assays vs proximal receptor signaling assays. 25 min.

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► GPCR Assays

Learn about several GPCR assays and which ones to use and why for your research and drug discovery and development program.

Find out what GPCR product types are available and discover assays including cAMP and calcium accumulation, β-arrestin recruitment, internalization, pharmacotrafficking, radioligand binding, and GTPyS assays to expedite your GPCR research and therapeutic development. Video covers GPCR families, targets, assay technology and protocol, and applications for target ID through QC lot release. 30 min.

View Related Products discoverx.com/gpcrs and Short Overview Recording 5 min.

Translocation

► Internalization Assays

Learn about PathHunter® Internalization assays developed to measure translocation of both single-pass and multi-pass receptors (like RTKs, checkpoint receptors and GPCRs) to intracellular endosomes.

Identify safer therapeutics with simple, quantitative cell-based assays for measuring receptor internalization. 13 min.

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► Pharmacotrafficking Assays

Learn about PathHunter® pharmacotrafficking assays to identify small molecule compounds that rescue disease relevant mutant membrane proteins like GPCRs, ion channels, and transporters.

Discover how these quantitative, antibody- and image-free cell-based assays can be used to screen compounds (called pharmacological chaperones) that stabilize mutated membrane proteins allowing for their proper trafficking to the cell membrane. Applications discussed include rescuing conditions like retinitis pigmentosa, obesity, diabetes, cystic fibrosis, and cardiac arrhythmias. 30 min.

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Custom Capabilities

► Custom Development Capabilities

Learn about Eurofins DiscoverX cell line engineering, assay development, and recombinant enzyme production custom capabilities.

Find out more about cell line engineering with CRISPR/Cas9; custom bioassay development & qualification; and projects for cytotoxicity, immune-oncology, GPCRs, target engagement & more. Discover options for protein expression and purification through custom assay development for your protein of interest. 23 min.

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