[User Manual] ProLabel® and ProLink™ Mammalian Cloning Vectors

[User Manual] ProLabel® and ProLink™
Mammalian Cloning Vectors
Version:
102416

File Name/Number:
70-385

Year:
2016

pCMV Mammalian cloning vectors are intended for cloning your protein of interest (e.g. membrane protein or intracellular protein) with ePL, PK1 or PK2, as a N- or C-terminal tag, and subsequently transfecting into a parental cell line expressing an EA-tagged reporter protein (Note: in some assay formats the cells only express an ED protein and the EA protein is added to the assay at the time of detection).

ePL and PK tags have related but distinct amino acid sequences, each of which has been optimized for different applications. Refer to the table below for additional information for each cloning vector.