[User Manual] PathHunter® GPCR Bioassay Kit
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PathHunter® GPCR Bioassay kits are ready to use kits that contain everything necessary to perform a functional assay with live cells, without any continuous cell culture. This kit has been designed to rapidly detect the potency of your biologic or the presence of neutralizing antibodies against the biologic. The Bioassay kits include single use vials of frozen cells expressing the target GPCR, chemiluminescent detection reagents, control agonist, optimized cell plating reagent, protein dilution buffer and plates. Simply thaw and plate the pre-validated cells and treat with test reagents within 24 to 48 hours. Assays have been designed for 96-well and 384-well plate formats.