[DOT 2022] SPRINTer Platform: Detection of Endogenous Protein Turnover and Target Engagement of Targeted Degraders

[DOT 2022] SPRINTer Platform: Detection of Endogenous Protein Turnover and Target Engagement of Targeted Degraders
Version:
21389

File Name/Number:
DOT

Year:
2022

 

An assay platform that robustly and sensitively quantifies the kinetics of endogenous protein turnover and target engagement is crucial for discovery of disease-relevant therapeutic agents. This need is particularly relevant for a new class of therapeutics known as protein degraders, such as PROTACs that target specific disease-relevant proteins for degradation by the cellular ubiquitin-proteasome system.