[DOT 2019] Real-Time Kinetic Analysis of GPCR Signaling in Stable PathHunter Cell Lines

[DOT 2019] Real-Time Kinetic Analysis of
GPCR Signaling in Stable PathHunter Cell Lines
File Name/Number:
DOT 2019

Year:
2019

Presented by:  Montana Molecular
 
  • Eurofins DiscoverX stable cell lines are widely used tools in GPCR drug discovery, providing endpoint assays for G-protein signaling, GPCR internalization, and β-arrestin recruitment.
  • Montana Molecular offers genetically-fluorescent biosensors to detect cAMP, DAG, PIP2 , Ca2+, cGMP, β-arrestin, and cell stress.
  • The fluorescent sensors can be used effectively in both PathHunter® and cAMP Hunter® CHO-K1 cell lines to monitor signaling kinetics of G-protein and β-arrestin pathways in live cells, in real time.
  • A new method of kinetic analysis provides a robust kinetic parameter (kTau), which simplifies the quantification of agonist activity and bias at particular GPCRs, and could provide a new method for studying G-protein and/or β-arrestin recruitment in opioid, angiotensin II, and vasopressin receptor cell lines.