A representative standard curve is shown in the graph. HitHunter cGMP has been designed to exhibit robust and reliable assay performance as defined by the following parameters: EC50 < 20nM; Z'factor > 0.75 [click graph to enlarge].
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The HitHunter cGMP assay can be used to identify PDE inhibitors. 12.5 U/well PDE V enzyme was used in this assay, dose dependent inhibition of the enzyme was observed. IBMX yielded an EC50 of 1.62 uM [click graph to enlarge].
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