Target Engagement Assays

Universal Cell-based Compound-target Binding Assays

Target engagement assays measure compound binding to a specific protein target. In a cellular environment, these assays monitor compound cellular penetration and compound-target interaction to enable the assessment of compound efficacy and confirmation of mode-of-action.

InCELL cell-based compound-target engagement assays provide the ability to confirm compound cell entry and protein target binding, screen and rank inhibitors, validate hits identified in biochemical assays, and measure cellular EC50 values all in the native cellular environment. These simple binding assays require no custom chemical tracers nor antibody reagents and provide a convenient solution when functional assays are difficult or unavailable.

Access two leading Eurofins DiscoverX® assay platforms, InCELL Pulse™ and InCELL Hunter™ — both based on the industry validated EFC technology with similar underlying principles, but crucial differences that suit them for different protein targets. The InCELL Pulse platform is built on the principle of protein thermal stability, while InCELL Hunter is built on the principle of protein stabilization related to protein turnover or interaction disruption.

InCELL target engagement assays are a perfect complement to Targeted Protein Degradation (TPD) programs that allow you interrogate and optimize target-binding portion. Discover the power of the InCELL assays and take advantage of stable cell lines, ready-to-use kits, and a do-it-yourself, starter kit perfect for creating your own compound-target engagement assay.

Product Highlights
  • Biologically Relevant - Obtain quantitative cellular compound entry and target binding data for more confident drug discovery decisions
  • Easy-to-Use - Simple, homogeneous protocol to easily measure compound binding to your protein target with a chemiluminescent readout
  • High-Throughput Amenable - Easily automate assays to screen multiple compounds accurately
  • Quick & Thorough Protocol - Rapidly make your own target engagement cell-based assays in less than 2 weeks following the detailed, step-by-step protocol

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

SHOP PRODUCTSVIEW VIDEOREAD BLOGKINASE PRODUCT LIST

Products

Eurofins DiscoverX offers two target engagement assay platforms, InCELL Pulse and InCELL Hunter. The InCELL Hunter platform offers researchers cell lines, ready-to-use kits and profiling services for specific targets. The InCELL Pulse platform offers researchers ready-to-use kits for specific targets and a do-it-yourself, starter kit perfect for creating your own compound-target engagement assay.

  • InCELL Hunter Cell Lines – Stable cell lines confirmed for past 10 passages and rigorously and continuously validated for accurate pharmacology through Eurofins Discovery services with billions of data points screened.
  • InCELL Hunter eXpress Kits – Complete assay-ready kits with specific cell line, optimized reagents, and plates for quick Enzyme Fragment Complementation (EFC) based chemiluminescent detection.
  • InCELL Pulse Starter Kit – Create your own cell-based, target engagement assays with reagents and protocol for the design and development of InCELL Pulse assays for the measurement of inhibitor cellular potency against user-defined protein targets of interest.
  • InCELL Detection Kit – Measure compound binding to a specific protein target in InCELL Hunter and InCELL Pulse cell lines